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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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MYU K.K.
Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 26, Number 8 (2014)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 607-614
S&M1027 Research Paper of Special Issue https://doi.org/10.18494/SAM.2014.1023 Published: October 21, 2014 Assembly of Poly(vinyl alcohol) and DNA via Hydrogen Bonds Induced by High Hydrostatic Pressurization [PDF] Tsuyoshi Kimura, Akira Okuno, Yuichi Ohya and Akio Kishida (Received January 29, 2014; Accepted July 16, 2014) Keywords: high hydrostatic pressure, DNA, poly(vinyl alcohol), hydrogen bond, assembly
According to our previous research, it was found that the assemblies of poly(vinyl alcohol) (PVA) and DNA, such as nanoparticles, microparticles and hydrogels, were formed through a high hydrostatic pressure process (10000 atm, 10 min) and could be applicable for gene delivery. In the present study, the PVA-DNA assembly was characterized by various methods, such as agarose gel electrophoresis, melting point and circular dichroism measurements, atomic force microscopy (AFM) observation and nuclease digestion assay. From agarose gel electrophoresis and melting point measurement of the PVA-DNA assembly, it was clarified that the interaction between them was hydrogen bonding. The B-type form of DNA of the PVA-DNA assembly was shown by circular dichroism measurement. The increase in width of DNA in the PVA-DNA assembly was observed by AFM. The increase in nuclease resistance of the PVA-DNA assembly was shown. From these results, it was suggested that the PVA was entwined with the DNA duplex via hydrogen bonds under high hydrostatic pressure.
Corresponding author: Tsuyoshi Kimura Cite this article Tsuyoshi Kimura, Akira Okuno, Yuichi Ohya and Akio Kishida, Assembly of Poly(vinyl alcohol) and DNA via Hydrogen Bonds Induced by High Hydrostatic Pressurization, Sens. Mater., Vol. 26, No. 8, 2014, p. 607-614. |
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