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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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MYU K.K.
Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 26, Number 10 (2014)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 737-744
S&M1037 Research Paper https://doi.org/10.18494/SAM.2014.1026 Published: December 22, 2014 Signal Enhancement of Indirect-Competitive Metallothionein Immunosensor by Horseradish-Peroxidase-Induced Precipitation of Diaminobenzidine Derivatives [PDF] Namsoo Kim (Received February 17, 2014; Accepted September 12, 2014) Keywords: signal enhancement, indirect-competitive metallothionein sensor, horseradish peroxidase (HRP)-induced precipitation, diaminobenzidine derivatives
An indirect-competitive quartz crystal microbalance immunosensor is well acknowledged as a tool for biological detection. As a method of improving sensitivity, horseradish-peroxidase-induced precipitation of 3,3’-diaminobenzidine (DAB) derivatives to the sensor surface, which was confirmed by field emission-scanning electron microscopy imaging, was conducted using metallothionein (MT) as a model analyte. The sensor signal was enhanced up to 5.3-fold when the DAB g/ml. Linearity in response signal was found in the MT range of 0.05–1 ng/ml, with the limit of detection of 50 pg/ml. The present biosensor can serve as a highly sensitive detection tool for biomarkers of physiological importance.
Corresponding author: Namsoo Kim Cite this article Namsoo Kim, Signal Enhancement of Indirect-Competitive Metallothionein Immunosensor by Horseradish-Peroxidase-Induced Precipitation of Diaminobenzidine Derivatives, Sens. Mater., Vol. 26, No. 10, 2014, p. 737-744. |
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