pp. 377-382
S&M574 Research Paper of Special Issue Published: 2004 Urease-Based Heavy Metal Ion Sensing Using a Silicon Transducer [PDF] Atsushi Seki and Satoshi Nomura (Received June 18, 2004; Accepted November 11, 2004) Keywords: light-addressable potentiometric sensor (LAPS), urease, inhibition, heavy metal ion
A heavy metal ion sensor based on urease inhibition and a semiconductor transducer were fabricated and characterized. Urease (EC 3.5.1.5) was reacted with copper (II) ion and mercury (II) ion, and the activity of the enzyme was measured. The pH change resulting from the enzyme reaction was monitored using a light-addressable potentiometric sensor (LAPS). This device detected the alkalization of the solution due to the enzymatic reaction, and the alkalization depended on the heavy metal ion concentration. The alkalization rates were approximately linear from 10 M to 30 M and from 1 M to10 M for copper and mercury, respectively. In addition, urease was immobilized on the electrode surface. The sensor responded to the addition of copper, and the enzyme membrane was restored by treatment with EDTA.
Corresponding author: Atsushi SekiCite this article Atsushi Seki and Satoshi Nomura, Urease-Based Heavy Metal Ion Sensing Using a Silicon Transducer, Sens. Mater., Vol. 16, No. 8, 2004, p. 377-382. |