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Vol. 34, No. 8(3), S&M3042

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Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.

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Sensors and Materials, Volume 27, Number 11 (2015)
Copyright(C) MYU K.K.
pp. 1123-1130
S&M1149 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2015.1211
Published: December 9, 2015

A Fluorometric Biochemical Gas Sensor (Biosniffer) for Acetaldehyde Vapor Based on Catalytic Reaction of Aldehyde Dehydrogenase [PDF]

Yuki Suzuki, Ming Ye, Kumiko Miyajima, Takahiro Arakawa, Shin-ichi Sawada, Hiroyuki Kudo, Kazunari Akiyoshi and Kohji Mitsubayashi

(Received May 27, 2015; Accepted September 24, 2015)

Keywords: fiber-optic, biosensor, acetaldehyde, aldehyde dehydrogenase, gas sensor

A fiber-optic biochemical gas sensor (biosniffer) for the continuous monitoring of acetaldehyde (AA) vapor was constructed and tested. The biosniffer was constructed using a flow cell with an aldehyde dehydrogenase (ALDH) membrane onto a fiber-optic reduced nicotinamide adenine dinucleotide (NADH) measurement system. AA vapor was measured as fluorescence of NADH produced by the enzymatic reaction of ALDH. A phosphate buffer that contained oxidized nicotinamide adenine dinucleotide (NAD+) was circulated into the flow cell to rinse NADH and the excessive substrates from the optode. A 340 nm ultraviolet light-emitting diode (UV-LED) was employed as an excitation source. The fluorescence of NADH (λ = 491 nm) was measured coaxially with a spectrometer. Characterization of the biosniffer was carried out using a standard AA measurement system. As a result, the typical response is between 0.1 to 5.0 ppm AA, which included the 8-h reference exposure level (0.16 ppm). The biosniffer is expected to be useful in the measurement of environmental AA.

Corresponding author: Kohji Mitsubayashi


Cite this article
Yuki Suzuki, Ming Ye, Kumiko Miyajima, Takahiro Arakawa, Shin-ichi Sawada, Hiroyuki Kudo, Kazunari Akiyoshi and Kohji Mitsubayashi, A Fluorometric Biochemical Gas Sensor (Biosniffer) for Acetaldehyde Vapor Based on Catalytic Reaction of Aldehyde Dehydrogenase, Sens. Mater., Vol. 27, No. 11, 2015, p. 1123-1130.



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