Biochips have been the center of attention in biological research because they can be employed in improving various genetic analysis techniques, such as reducing both analysis time and the quantity of sample required. In particular, biochips have been extensively employed in polymerase chain reaction (PCR) experiments, in which sensors are required to measure the temperature. This study proposed a temperature sensor chip that enables the implementation of a resistance temperature detector made of copper on a printed circuit board (PCB). The calibration of each sensor chip was investigated with either a 2-point or a 5-point fitting, and we determined that 2-point calibration was sufficient for application to PCR. The calibrated resistance detector chip can accurately measure the temperature at room temperature, 55, 72, 85, and 95 °C in a water bath. The 2-point calibration resulted in less than a 0.52 °C error in the reported temperature.