1-Methoxy 5-methylphenazinium methyl sulphate (1-MeO-PMS) was adsorbed on the disk plane of a plastic formed carbon (PFC) rod electrode (3 mm in diameter). PFC is a novel carbon material prepared by mixing various amounts of pure graphite with an organic binder and pyrolyzing to “glassy carbon” at moderate final temperatures of 1000–1400 ℃. The prepared 1-MeO-PMS/PFC electrode exhibited a sufficient electrocatalytic activity for dihydronicotinamide adenine dinucleotide (NADH) oxidation. At an applied potential of −0.05 V vs Ag/AgCl at pH 7.0, the 1-MeO-PMS/PFC electrode showed a fast (<5 s) and linear anodic current response to NADH over the concentration range of 1 to 100 μM with a limit of detection of 0.8 µM (S/N = 3). NAD+-dependent glucose dehydrogenase (GDH) was further adsorbed on the 1-MeO-PMS/PFC electrode for glucose sensing. In the presence of 10 mM NAD+ in 0.1 M phosphate buffer (pH 8.0), the prepared GDH/1-MeO-PMS/PFC electrode showed a fast (<10 s) and linear current response to glucose over the concentration range of 10 to 100 μM with a limit of detection of 7 μM (S/N = 3).