pp. 2929-2940
S&M2312 Research Paper https://doi.org/10.18494/SAM.2020.2959 Published: September 8, 2020 Label-free Identification of Bacteria Species Based on Divergence Angle Distribution of Light Scattering Patterns [PDF] Jinsil Han, Jun Hyeok Jeong, Seul-Lee Lee, Heejin Jeong, Young-Mog Kim, and Yong Wook Lee (Received June 12, 2020; Accepted July 29, 2020) Keywords: bacteria identification, light scattering, scattering pattern, divergence angle, Gaussian distribution
In this paper, we propose a facile scheme for label-free bacteria discrimination by utilizing the divergence angles (DAs) of the light scattering patterns (LSPs) of bacteria colonies. We compared three species of bacteria, Escherichia coli, Salmonella typhimurium, and Listeria monocytogenes, in terms of the DAs of the forward LSPs of their colonies by using the proposed scheme. The sizes of the cultured bacterial colonies used for comparison ranged from 0.8 to 2.2 mm for all three species. First, to obtain their LSPs, a low-power green laser of 532 nm illuminated each bacterial colony. Then, the sizes of the LSPs were estimated from the acquired colony scattering patterns. The DAs of the LSPs were calculated using the measured colony sizes and LSP sizes. Experimental results show that there exists an optimal distribution range of the DA for each bacterial species. We believe that this new identification scheme of pathogens using the specific DA distribution range will be beneficially utilized for a simple verification of conventional bacteria discrimination methods.
Corresponding author: Yong Wook LeeThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Jinsil Han, Jun Hyeok Jeong, Seul-Lee Lee, Heejin Jeong, Young-Mog Kim, and Yong Wook Lee, Label-free Identification of Bacteria Species Based on Divergence Angle Distribution of Light Scattering Patterns, Sens. Mater., Vol. 32, No. 9, 2020, p. 2929-2940. |