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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
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Sensors and Materials, Volume 34, Number 3(1) (2022)
Copyright(C) MYU K.K.
pp. 937-949
S&M2864 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2021.3585
Published in advance: October 27, 2021
Published: March 3, 2022

Method for Controlling Intracellular Protein Activity Using Photoresponsive Nanoparticles [PDF]

Shuhei Murayama, Noriaki Kono, Takashi Takaki, and Masaru Kato

(Received August 11, 2021; Accepted October 12, 2021)

Keywords: photoresponsive nanoparticles, gel mesh structure, encapsulated proteins, controlled release, protein function control

Protein levels in cells can be controlled by delivering and releasing the target protein to a cell. Herein, we report the preparation of photoresponsive nanoparticles with different protein loadings for application as protein delivery vehicles. The nanoparticles were prepared by radical polymerization using an X-shaped photoresponsive linker (Mw = 2000) and a cationic linker. The incorporation of a fluorescent polysaccharide (FITC-Dex) or protein (caspase 3) into the nanoparticles did not create significant differences in the physical properties of the resultant nanoparticles; they were ~150 nm in size with weak positive zeta potentials. These new photoresponsive nanoparticles can encapsulate and release various protein-containing compounds with different molecular weights (3–250 kDa). In addition, the amount of compound released from the nanoparticles can be tailored by altering the amount of compound encapsulated in the nanoparticles. In this study, nanoparticles containing cationic units were introduced into cells via endocytosis. We controlled the activity of the nanoparticle-released proteins within HeLa cells. Specifically, adjusting the protein loading in the nanoparticles enabled control over the activity of the proteins released into the cell, and thus the level of protein function, which provides a powerful tool for elucidating the functions of proteins.

Corresponding author: Masaru Kato


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This work is licensed under a Creative Commons Attribution 4.0 International License.

Cite this article
Shuhei Murayama, Noriaki Kono, Takashi Takaki, and Masaru Kato, Method for Controlling Intracellular Protein Activity Using Photoresponsive Nanoparticles, Sens. Mater., Vol. 34, No. 3, 2022, p. 937-949.

Supporting information
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