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Vol. 32, No. 8(2), S&M2292

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Online: ISSN 2435-0869
Sensors and Materials
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Sensors and Materials, Volume 34, Number 3(1) (2022)
Copyright(C) MYU K.K.
pp. 971-985
S&M2867 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2022.3618
Published in advance: January 5, 2022
Published: March 3, 2022

Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification [PDF]

Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, and Hizuru Nakajima

(Received September 3, 2021; Accepted November 18, 2021)

Keywords: fluorescence microplate reader, LAMP, ITO glass heater, on-site nucleic acid test, variety discrimination

We developed a portable fluorescence microplate reader equipped with an indium tin oxide (ITO) glass heater for nucleic acid testing based on loop-mediated isothermal amplification (LAMP). The components used for detection (e.g., LEDs, optical filters, the microwell plate, the ITO glass heater, and photodiodes) are arranged in parallel on a straight line. This optical placement allows us to simultaneously heat nine sample solutions and monitor the fluorescence intensity amplified by the LAMP reaction despite the analysis instrument being handheld. This simultaneous monitoring was demonstrated by the LAMP reaction using positive and negative controls. In addition, the microplate reader was applied to the variety discrimination of rice, and Koshihikari rice was successfully discriminated from four types of milled rice using the microplate reader. The developed microplate reader will be useful for on-site nucleic acid testing because it can be operated using batteries and is portable.

Corresponding author: Kazuhiro Morioka, Hizuru Nakajima


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Cite this article
Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, and Hizuru Nakajima, Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification, Sens. Mater., Vol. 34, No. 3, 2022, p. 971-985.



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