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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 34, Number 3(1) (2022)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 971-985
S&M2867 Research Paper of Special Issue https://doi.org/10.18494/SAM.2022.3618 Published in advance: January 5, 2022 Published: March 3, 2022 Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification [PDF] Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, and Hizuru Nakajima (Received September 3, 2021; Accepted November 18, 2021) Keywords: fluorescence microplate reader, LAMP, ITO glass heater, on-site nucleic acid test, variety discrimination
We developed a portable fluorescence microplate reader equipped with an indium tin oxide (ITO) glass heater for nucleic acid testing based on loop-mediated isothermal amplification (LAMP). The components used for detection (e.g., LEDs, optical filters, the microwell plate, the ITO glass heater, and photodiodes) are arranged in parallel on a straight line. This optical placement allows us to simultaneously heat nine sample solutions and monitor the fluorescence intensity amplified by the LAMP reaction despite the analysis instrument being handheld. This simultaneous monitoring was demonstrated by the LAMP reaction using positive and negative controls. In addition, the microplate reader was applied to the variety discrimination of rice, and Koshihikari rice was successfully discriminated from four types of milled rice using the microplate reader. The developed microplate reader will be useful for on-site nucleic acid testing because it can be operated using batteries and is portable.
Corresponding author: Kazuhiro Morioka, Hizuru Nakajima  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Ryo Ishii, Kazuhiro Morioka, Takuya Mizumoto, Natsumi Yamasaki, Akihide Hemmi, Atsushi Shoji, Hiroya Murakami, Norio Teshima, Tomonari Umemura, Katsumi Uchiyama, and Hizuru Nakajima, Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification, Sens. Mater., Vol. 34, No. 3, 2022, p. 971-985. |
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