Many office workers are exposed to fluorescent light from mid-morning to after dark. The changes in the amount of light in the natural world, from bright during the day to dark at night, play an important role in maintaining a regular daily rhythm. In this study, we evaluated the effects of LED lighting equipped with a circadian rhythm control function, Lavigo, by comparing it with conventional fluorescent lighting on students’ sleep quality and stress. Lavigo’s Visual Timing Light (VTL) function adjusts the color and brightness of light according to the biological rhythm of about 24 h cycles. We measured the sleep state using Fitbit and collected subjective responses through a stress-checking questionnaire. A salivary amylase monitor was used to measure objective stress values from a medical perspective. No significant difference in stress values was observed during the experiment. However, only during the week that Lavigo was used, a certain subject showed a significant decrease in waking time during sleep, and another subject showed an increase in deep sleep. It was also found that these subjects tended to lead an irregular life, such as staying up until midnight.