In view of the backward traditional training methods and the shortage of training funds in college sports, an agility training system based on a wireless mesh network is developed and evaluated. The network structure, initialization, and root node selection of the mesh network are described in detail. In the mesh network training scenario, the network topology is examined, and the response time and packet loss rate of the mesh network nodes are tested to demonstrate that the ad hoc network can transmit real-time data within the response time. The experimental data show that when the number of hops is n ≤ 2 and the response time of the mesh network nodes is less than 300 ms, the packet loss rate is 0%. The wireless mesh network solves the problem that the traditional Wi-Fi network is limited by the communication distance. It also allows more lower-level computer nodes to access the network and expands the training coverage.