Young Researcher Paper Award 2023
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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 35, Number 11(4) (2023)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 3863-3870
S&M3455 Research Paper of Special Issue https://doi.org/10.18494/SAM4371 Published: November 30, 2023 Development of Observation Method for Isoflavones Secreted by Soybean Roots in Rhizobox Using Sheets with Immobilized Bovine Serum Albumin [PDF] Takeshi Onodera, Ayako Ogawa, Haruna Miyazaki, Nozomu Sakurai, and Akifumi Sugiyama (Received March 6, 2023; Accepted September 12, 2023) Keywords: bovine serum albumin, soybeans, isoflavone, fluorescence quenching, imaging, rhizobox
A sensing method was developed to qualitatively evaluate the distribution of soybean isoflavones secreted by roots of soybeans transplanted into a simplified rhizobox by transferring them onto pieces of glass microfiber filter (sheets) with immobilized bovine serum albumin (BSA). To take advantage of fluorescence quenching by the interaction between BSA and soybean isoflavones, BSA was chemically bonded onto the sheets. The sheets with immobilized BSA were brought into contact with a nylon mesh (1 µm mesh aperture) of a simplified rhizobox, and secretions from soybean roots were transferred to the sheets. Fluorescence images before and after contact were captured with an electron-multiplying CCD camera to visualize and analyze fluorescence quenching. The results indicated that soybean isoflavone was spread within 2 to 3 mm from the roots.
Corresponding author: Takeshi Onodera  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Takeshi Onodera, Ayako Ogawa, Haruna Miyazaki, Nozomu Sakurai, and Akifumi Sugiyama, Development of Observation Method for Isoflavones Secreted by Soybean Roots in Rhizobox Using Sheets with Immobilized Bovine Serum Albumin, Sens. Mater., Vol. 35, No. 11, 2023, p. 3863-3870. |
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