S&M3455 Research Paper of Special Issue
Published: November 30, 2023
Development of Observation Method for Isoflavones Secreted by Soybean Roots in Rhizobox Using Sheets with Immobilized Bovine Serum Albumin [PDF]
Takeshi Onodera, Ayako Ogawa, Haruna Miyazaki, Nozomu Sakurai, and Akifumi Sugiyama
(Received March 6, 2023; Accepted September 12, 2023)
Keywords: bovine serum albumin, soybeans, isoflavone, fluorescence quenching, imaging, rhizobox
A sensing method was developed to qualitatively evaluate the distribution of soybean isoflavones secreted by roots of soybeans transplanted into a simplified rhizobox by transferring them onto pieces of glass microfiber filter (sheets) with immobilized bovine serum albumin (BSA). To take advantage of fluorescence quenching by the interaction between BSA and soybean isoflavones, BSA was chemically bonded onto the sheets. The sheets with immobilized BSA were brought into contact with a nylon mesh (1 µm mesh aperture) of a simplified rhizobox, and secretions from soybean roots were transferred to the sheets. Fluorescence images before and after contact were captured with an electron-multiplying CCD camera to visualize and analyze fluorescence quenching. The results indicated that soybean isoflavone was spread within 2 to 3 mm from the roots.Corresponding author: Takeshi Onodera
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Cite this article
Takeshi Onodera, Ayako Ogawa, Haruna Miyazaki, Nozomu Sakurai, and Akifumi Sugiyama, Development of Observation Method for Isoflavones Secreted by Soybean Roots in Rhizobox Using Sheets with Immobilized Bovine Serum Albumin, Sens. Mater., Vol. 35, No. 11, 2023, p. 3863-3870.