Young Researcher Paper Award 2023

Notice of retraction
Vol. 34, No. 8(3), S&M3042

Notice of retraction
Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Sensors and Materials
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PACKTEST for L-Glutamate Quantification: Development of On-site and High-throughput Analytical Kits using L-Glutamate Oxidase Mutant

Keita Murai, Hiroki Yamaguchi, Satoru Furuuchi, Kazutoshi Takahashi, Uno Tagami, Moemi Tatsumi, Toshimi Mizukoshi, Hiroshi Miyano, Shuntaro Okauchi, and Masayuki Sugiki

(Received August 21, 2023; Accepted February 27, 2027)

Keywords: L-glutamate, colorimetry, glutamate oxidase, enzyme-based sensing, amino acid analysis

L-glutamate is an umami component found in various foods, and L-glutamate oxidase (LGOX, EC is a FAD-dependent oxidoreductase that catalyzes the oxidative deamination of L-glutamate. A recently reported single-chain LGOX mutant from Streptomyces sp. X-119-6 is a potential material for L-glutamate sensors because this mutant is easier to prepare than the wild type. Herein, we report the development of a simple analytical kit using this LGOX mutant for the on-site quantification of L-glutamate. The kit named PACKTEST was developed using a colorimetric method to estimate the L-glutamate concentration in the range of 1–50 mg/L through visual comparison of the resulting sample color with a standard color sequence. In addition, the PACKTEST kit was combined with a portable photometer for the spectrophotometric quantification of L-glutamate in the range of 0.5–12.0 mg/L. It was used to quantify L-glutamate in commercially available soy sauces, and the results were in good agreement (r = 0.97) with the values obtained using post-column derivatized ion-exchange chromatography. Furthermore, we loaded and dried all the reagents, including enzymes, on a 96-well plate to prepare an enzyme-based sensing device, which showed potential for the high-throughput quantification of L-glutamate.

Corresponding author: Keita Murai and Hiroki Yamaguchi

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