Young Researcher Paper Award 2023

Notice of retraction
Vol. 34, No. 8(3), S&M3042

Notice of retraction
Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
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Development of Portable Multi-fluorescence Detection System Using Indium Tin Oxide Heater for Loop-mediated Isothermal Amplification

Ryo Ishii, Sota Hirose, Shoji Yamamoto, Kazuhiro Morioka, Akihide Hemmi, and Hizuru Nakajima

(Received August 31, 2023; Accepted January 15, 2024)

Keywords: fluorescence detection, ITO transparent heater, LAMP, on-site gene testing

We developed a portable multi-fluorescence detection system for loop-mediated isothermal amplification (LAMP) using an indium tin oxide (ITO) transparent glass heater by improving a previously developed device. By changing the optical design and the material of the heating part, the quantitative performance and the stability of temperature control were improved. We demonstrated the basic performance of the developed device for genetic tests using LAMP by conducting discrimination tests for human genome DNA. We also showed that quantitative analysis of human genomic DNA is possible at initial DNA concentrations of 2.11×102–2.11×104 ng/µL using the developed device. This genetic testing device is smaller and lighter (60 mm × 90 mm × 70 mm, 178 g) than conventional testing devices, giving it considerable potential to facilitate rapid and efficient on-site genetic testing.

Corresponding author: Shoji Yamamoto and Hizuru Nakajima

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