pp. 3189-3199
S&M3726 Research Paper of Special Issue https://doi.org/10.18494/SAM4635 Published: August 2, 2024 Development of Portable Multi-fluorescence Detection System Using Indium Tin Oxide Heater for Loop-mediated Isothermal Amplification [PDF] Ryo Ishii, Sota Hirose, Shoji Yamamoto, Kazuhiro Morioka, Akihide Hemmi, and Hizuru Nakajima (Received August 31, 2023; Accepted January 15, 2024) Keywords: fluorescence detection, ITO transparent heater, LAMP, on-site gene testing
We developed a portable multi-fluorescence detection system for loop-mediated isothermal amplification (LAMP) using an indium tin oxide (ITO) transparent glass heater by improving a previously developed device. By changing the optical design and the material of the heating part, the quantitative performance and the stability of temperature control were improved. We demonstrated the basic performance of the developed device for genetic tests using LAMP by conducting discrimination tests for human genome DNA. We also showed that quantitative analysis of human genomic DNA is possible at initial DNA concentrations of 2.11×102–2.11×104 ng/µL using the developed device. This genetic testing device is smaller and lighter (60 mm × 90 mm × 70 mm, 178 g) than conventional testing devices, giving it considerable potential to facilitate rapid and efficient on-site genetic testing.
Corresponding author: Shoji Yamamoto and Hizuru NakajimaThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Ryo Ishii, Sota Hirose, Shoji Yamamoto, Kazuhiro Morioka, Akihide Hemmi, and Hizuru Nakajima, Development of Portable Multi-fluorescence Detection System Using Indium Tin Oxide Heater for Loop-mediated Isothermal Amplification, Sens. Mater., Vol. 36, No. 8, 2024, p. 3189-3199. |