Young Researcher Paper Award 2023
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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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English 日本語
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MYU K.K.
Sensors and Materials
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The Art of Writing Scientific Papers
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 36, Number 3(1) (2024)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 811-822
S&M3563 Research Paper of Special Issue https://doi.org/10.18494/SAM4811 Published: March 8, 2024 Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection [PDF] Takeshi Onodera, Taiki Ajisaka, Ayako Ogawa, Yuko Hashiguchi, Nozomu Sakurai, and Akifumi Sugiyama (Received November 30, 2023; Accepted January 10, 2024) Keywords: daidzein, immunochromatography, surface plasmon resonance, indirect competitive method, monoclonal antibody
In this study, we aimed to detect daidzein secreted in the soybean rhizosphere using an indirect competitive immunochromatographic assay. A specific anti-daidzein monoclonal antibody was obtained through the establishment of a hybridoma. The antibody exhibited a detection limit of 5 ppb daidzein and showed strong cross-reactivity with daidzein, as determined by an indirect competitive method using a surface plasmon resonance sensor. Through the findings, a unique binding pattern differing from those of genistein and other saponins was observed. Enhanced sensitivity with the ability to detect daidzein concentrations as low as 10 nM (2.5 ng/mL) was achieved using immunochromatographic test strips with modified gold nanoparticles
Corresponding author: Takeshi Onodera  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Takeshi Onodera, Taiki Ajisaka, Ayako Ogawa, Yuko Hashiguchi, Nozomu Sakurai, and Akifumi Sugiyama, Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection, Sens. Mater., Vol. 36, No. 3, 2024, p. 811-822. |
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