pp. 207-218
S&M839 Research Paper of Special Issue https://doi.org/10.18494/SAM.2011.730 Published: July 11, 2011 Copper-Binding Peptide-Fragment-Containing Membrane as a Biocatalyst Prepared by Radiation-Induced Graft Polymerization [PDF] Tadashi Okobira, Takashi Kadono, Tomoko Kagenishi, Ken Yokawa, Tomonori Kawano and Kazuya Uezu (Received October 19, 2010; Accepted December 13, 2010) Keywords: glycine-glycine-histidine (GGH), chemiluminescence, superoxide, aromatic monoamine, tyramine, polymer brush, biocatalyst, computational chemistry
The GlyGlyHis (GGH) peptide was introduced to glycidyl methacrylate (GMA)-grafted porous hollow fiber membrane made of polyethylene by radiation-induced graft polymerization. The GGH density in the membrane was 0.352 mmol/g-membrane. The copper sulfate solution was permeated outward through the GGH peptide-containing membrane, and Cu(II) was adsorbed on the membrane. Chemiluminescence between cypridina luciferin analog (CLA) and superoxide from the catalytic reaction with H2O2 and tyramine on the membrane was measured, and the Cu-binding GGH peptide-containing membrane exhibited a very strong chemiluminescence response. Furthermore, we evaluated the molecular structure of the repeating unit model of the Cu-binding GGH peptide-containing polymer brush without trunk polyethylene by computational chemistry.
Corresponding author: Kazuya UezuCite this article Tadashi Okobira, Takashi Kadono, Tomoko Kagenishi, Ken Yokawa, Tomonori Kawano and Kazuya Uezu, Copper-Binding Peptide-Fragment-Containing Membrane as a Biocatalyst Prepared by Radiation-Induced Graft Polymerization, Sens. Mater., Vol. 23, No. 4, 2011, p. 207-218. |