Compact biosensors have been recognized as being useful for analyzing biomarkers such asprotein and gene products from the micro- or nano-liter scale of human samples for diagnosis ofhuman disease, and have led to the development of lab-on-a-chip or micro-total-analysis systems.Biosensor platforms allow near real-time detection and estimation of human disease indicators.Following a brief introduction of three elementary functions of biosensors—the molecularrecognition element, the transducer element, and the microfluidic control mechanism—this reviewfocuses on the current status of development of microfluidic control mechanisms for biosensors,and future technological advances. In this review, we highlight the recent experimental advancesin centrifuge-based microfluidic platforms (centrifugal microfluidics), which aim to increase abiosensor’s sensitivity and selectivity. Particular focus is placed on the application of hydrophobicmicropatterns to centrifugal microfluidics. Centrifugal microfluidics will render biomarkers morefield-practical and accessible to end-users in a cost-effective manner.