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S&M1473 Research Paper https://doi.org/10.18494/SAM.2018.1589 Published: January 12, 2018 Enhanced Fluorescent DNA Microarray Using Optical Interference Mirror Slide [PDF] Mitsuru Yasuda and Takuo Akimoto (Received March 2, 2017; Accepted June 1, 2017) Keywords: DNA microarray, fluorescence enhancement, optical interference
Enhanced fluorescence using an optical interference mirror (OIM) slide consisting of a metal film and a transparent dielectric thin film is used to improve the sensitivity of a DNA microarray. Twenty-one bases of a probe DNA are immobilized on the OIM slide fabricated with Ag and Al2O3, and 21 bases of target DNA, whose sequence is complementary to that of the probe DNA, are detected. After evaluating the hybridization specificity between the probe DNA and the target DNA, the sensitivity improvement is estimated. As a result, a 50-fold improvement can be achieved compared with a bare glass slide.
Corresponding author: Takuo AkimotoCite this article Mitsuru Yasuda and Takuo Akimoto, Enhanced Fluorescent DNA Microarray Using Optical Interference Mirror Slide, Sens. Mater., Vol. 30, No. 1, 2018, p. 59-66. |