pp. 437-445
S&M1784 Research Paper of Special Issue https://doi.org/10.18494/SAM.2019.2101 Published: February 18, 2019 Study of Optimal Real-time Polymerase Chain Reaction Chip by Comparison of Coating Reflective Materials [PDF] Ji-Soo Hwang, Bo-Jun Kim, Jong-Dae Kim, Yu-Seop Kim, Hye-Jeong Song, and Chan-Young Park (Received May 31, 2018; Accepted October 18, 2018) Keywords: real-time polymerase chain reaction, fluorescence detection, relative gap, microfluidic channel, PCR chip, lab-on-a-chip
The real-time polymerase chain reaction (RT-PCR) test chip employed in previous experiments used a matte black printed circuit board (PCB) substrate printed with a white silk legend on a heating pattern. However, when analyzing the fluorescence detection results, the chip needed to be replaced with a coating material with a high reflection efficiency because of a large variation in brightness. Therefore, in this work, chips using a PCB substrate coated with various kinds of materials are manufactured, and the relative gains of these chips are compared to select a chip showing the most optimal reflection efficiency. As a result, the reflection efficiency of the PCR chip using a silver-coated PCB substrate was found to be the best.
Corresponding author: Chan-Young ParkThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Ji-Soo Hwang, Bo-Jun Kim, Jong-Dae Kim, Yu-Seop Kim, Hye-Jeong Song, and Chan-Young Park, Study of Optimal Real-time Polymerase Chain Reaction Chip by Comparison of Coating Reflective Materials, Sens. Mater., Vol. 31, No. 2, 2019, p. 437-445. |