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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 31, Number 5(2) (2019)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 1635-1646
S&M1886 Research Paper of Special Issue https://doi.org/10.18494/SAM.2019.2284 Published: May 31, 2019 Compact Fluorescence Detection System for Polymerase Chain Reaction Chips [PDF] Deuk-Ju Lee, Ji-Soo Hwang, Ji-Seong Park, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, and Jong-Dae Kim (Received April 16, 2018; Accepted March 20, 2019) Keywords: DNA detection, PCR chip, fluorescence, optic, excitation, emission
In this paper, we propose a compact system for the fluorescence detection of polymerase chain reaction (PCR) chips. Various fluorescence detection methods have been proposed, but all require many optical components and optical distances. In contrast, in this paper, we propose a system for detecting fluorescence by attaching an LED and a photodiode directly to the reagent surface. The system is very compact and inexpensive because it requires only excitation and emission filters. To optimize the performance of the proposed system, LED and photodiode arrangements, selection of filters, and signal amplification circuits were investigated. The modification of the PCR protocol to obtain better results was also investigated. PCR amplification experiments on real DNA samples showed that the proposed system works well.
Corresponding author: Jong-Dae Kim  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Deuk-Ju Lee, Ji-Soo Hwang, Ji-Seong Park, Chan-Young Park, Hye-Jeong Song, Yu-Seop Kim, and Jong-Dae Kim, Compact Fluorescence Detection System for Polymerase Chain Reaction Chips, Sens. Mater., Vol. 31, No. 5, 2019, p. 1635-1646. |
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