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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
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Sensors and Materials, Volume 34, Number 2(3) (2022)
Copyright(C) MYU K.K.
pp. 835-841
S&M2856 Research Paper of Special Issue
https://doi.org/10.18494/SAM.2022.3644
Published in advance: January 6, 2022
Published: February 28, 2022

Design and Implementation of a Miniaturized Dual-wavelength UV Crosslinker [PDF]

Zhiyou Wang and Maojin Wang

(Received September 6, 2021; Accepted December 1, 2021)

Keywords: LED light source, Wi-Fi communication, uniform irradiation, palm size

To satisfy the requirement of microarray fabrication in optical sensing applications, we report a palm-sized UV LED crosslinker. By employing a microcontroller and the Wi-Fi communication technique, a sequence of different wavelengths, irradiation intensities, and crosslinking durations can be programmed by an Android app. To evaluate the irradiation uniformity of the crosslinker, we immobilized an array of fluorescent-labeled proteins on a silver substrate of high flatness by the reported crosslinker in 8 s and measured the fluorescence intensity throughout the array. For protein spots of the same concentration, the intensity difference is around 10% of the average value. The results show that the reported UV LED crosslinker can help to achieve the uniform and efficient immobilization of a protein microarray.

Corresponding author: Zhiyou Wang


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Cite this article
Zhiyou Wang and Maojin Wang, Design and Implementation of a Miniaturized Dual-wavelength UV Crosslinker, Sens. Mater., Vol. 34, No. 2, 2022, p. 835-841.



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