S&M2856 Research Paper of Special Issue
Published in advance: January 6, 2022
Published: February 28, 2022
Design and Implementation of a Miniaturized Dual-wavelength UV Crosslinker [PDF]
Zhiyou Wang and Maojin Wang
(Received September 6, 2021; Accepted December 1, 2021)
Keywords: LED light source, Wi-Fi communication, uniform irradiation, palm size
To satisfy the requirement of microarray fabrication in optical sensing applications, we report a palm-sized UV LED crosslinker. By employing a microcontroller and the Wi-Fi communication technique, a sequence of different wavelengths, irradiation intensities, and crosslinking durations can be programmed by an Android app. To evaluate the irradiation uniformity of the crosslinker, we immobilized an array of fluorescent-labeled proteins on a silver substrate of high flatness by the reported crosslinker in 8 s and measured the fluorescence intensity throughout the array. For protein spots of the same concentration, the intensity difference is around 10% of the average value. The results show that the reported UV LED crosslinker can help to achieve the uniform and efficient immobilization of a protein microarray.Corresponding author: Zhiyou Wang
This work is licensed under a Creative Commons Attribution 4.0 International License.
Cite this article
Zhiyou Wang and Maojin Wang, Design and Implementation of a Miniaturized Dual-wavelength UV Crosslinker, Sens. Mater., Vol. 34, No. 2, 2022, p. 835-841.