Electrochemical imaging with electrode arrays has been widely used to visualize chemicals and biomolecules. However, it is difficult to incorporate many sensors in a small area because of the large area of the leading connections and connector pads. To solve this problem, closed bipolar electrodes (BPEs) have been proposed as wireless electrochemical sensors, wherein many conductive materials are embedded in a thin film. However, it is laborious to prepare films with high-density BPEs. In this study, we demonstrate that commercially available anisotropic conductive films (ACFs) can be used as closed BPE films for electrochemiluminescence (ECL) imaging. Commercial ACFs are easily available without any fabrication equipment in a laboratory, which is a merit. The ACFs comprised conductive fibers embedded into a rubber film of 0.2 mm thickness. In the ACFs containing carbon fibers, the fiber diameter was 5–20 µm, and the fiber density was approximately 1 × 103 fibers/mm2. As the films were made of rubber, they bend and stretch easily, and thus are useful for bending and stretching applications during assays. During ECL imaging, redox compounds react at the cathodic poles of BPEs, and ECL chemicals are oxidized at the opposite poles. In this study, we characterized ACFs for ECL imaging. Additionally, we presented a novel configuration of electrodes for ECL imaging based on a BPE array film, where it is unnecessary to insert an electrode into the ECL chemical solution. Finally, a moving droplet was electrochemically visualized to study the crosstalk between BPEs. To the best of our knowledge, this is the first report concerning ECL imaging based on BPEs using commercially available ACFs. In the future, this strategy will be coupled with biosensing for cell analyses and immunoassays.