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Sensors and Materials, Volume 36, Number 5(1) (2024)
Copyright(C) MYU K.K.
pp. 1869-1879
S&M3641 Research Paper of Special Issue
Published: May 15, 2024

A Portable Platform for the Quantification of Vitamin D Levels Using Image Recognition and Paper-based Microfluidics [PDF]

Chen-Hsun Weng and Yu-Sheng Lin

(Received October 30, 2023; Accepted April 19, 2024)

Keywords: vitamin D, paper-based microfluidics, portable platform

Vitamin D deficiency has been associated with various diseases and adverse health outcomes, including diabetes, cardiovascular diseases, and cancer. Currently, the majority of vitamin D testing is conducted in large-scale laboratories upon the request of a physician as part of an annual panel of blood tests. In this paper, we present a novel paper-based microfluidic system that enables the rapid quantification of vitamin D levels on a portable platform. The system comprises a smartphone accessory, an app, and a test strip that allows for the colorimetric detection of 25-hydroxyvitamin D3 using a gold-nanoparticle-based immunoassay. We demonstrate that the system can accurately measure the physiological levels of 25-hydroxyvitamin D with an accuracy higher than 25 nM and a precision of 10 nM. We compared our system with the well-established ELISA test kits for serum samples of unknown concentration and demonstrated the equivalency of the obtained results.

Corresponding author: Yu-Sheng Lin

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Cite this article
Chen-Hsun Weng and Yu-Sheng Lin, A Portable Platform for the Quantification of Vitamin D Levels Using Image Recognition and Paper-based Microfluidics, Sens. Mater., Vol. 36, No. 5, 2024, p. 1869-1879.

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