In recent years, the conflict between human activities and the natural environment has led to global warming and the emergence of extreme weather events, prompting reflection on the climate adaptability of buildings. Historical and cultural districts often reflect the design and construction strategies of their era, which were shaped by the prevailing social environments and natural conditions, so they typically embody construction techniques tailored to address local climatic characteristics. Consequently, in this paper, we focus on 33 historic and cultural districts within Beijing’s old city, along with seven proposed historic and cultural districts, to retrieve land surface temperatures (LST) using Landsat remote sensing data and radiative transfer equations. A spatiotemporal analysis was conducted to investigate the variations in LST distribution across different historic and cultural districts and to characterize the internal temperature features within the historic and cultural districts of Beijing’s old city. Research indicates the following: (1) The Dashilan area boasts the highest average LST, while the Huangcheng area records the lowest. (2) Over the past six years, most historic and cultural districts have experienced a decline in LST. Notably, the most significant reductions in average LST have been observed in the areas west of Xinjiekou, Dongsi South, and from the 3rd to the 8th Street in Dongsi. (3) Within the historic and cultural districts, hot spots of LST are primarily concentrated in the residential areas with one-story traditional houses, while cold spots are predominantly found around water, cultural relics, and modern architectural structures.