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Sensors and Materials, Volume 21, Number 8 (2009)
Copyright(C) MYU K.K.
pp. 433-444
S&M782 Research Paper
Published: December 3, 2009

Fabrication of AucoreCo3O4shell/PAA/HRP Composite Film for Direct Electrochemistry and Hydrogen Peroxide Sensor Applications [PDF]

Xiaohua Chen, Huan Guo, Jing Yi and Jianqiang Hu

(Received January 16, 2009; Accepted July 10, 2009)

Keywords: biosensor, nanomaterial, polyacrylic acid, hydrogen peroxide, direct electrochemistry

A completely new biosensor composed of cube-shaped AucoreCo3O4shell nanoparticles (AucoreCo3O4shell), polyacrylic acid (PAA), and horseradish peroxidase (HRP) modified film electrode was fabricated for the first time. The biocompatibility and electrochemical properties of the resulting AucoreCo3O4shell-PAA-HRP composite film were studied by electrochemical impedance spectroscopy, UV-visible spectroscopy, and cyclic voltammetry. The UV-vis spectrum obtained suggests that HRP retains its native conformation in the modified film. The immobilized HRP shows a pair of quasi-reversible redox peaks at −0.31 V in 20 mM PBS (pH 7.0), and the biosensor shows a fast amperometric response to hydrogen peroxide with a linear range of 2.0×10−6 to 3.7×10−4 M. The kinetic parameters such as ks (electron transfer rate constant) and KM (Michaelis-Menten constant) are evaluated to be about 7.4 s−1 and 0.91 mM, respectively. These indicate that the cube-shaped AucoreCo3O4shell nanoparticles are an ideal candidate material for direct electrochemistry of redox proteins and for the construction of related enzyme biosensors, and that they may find potential applications to biomedical, food, and environmental analyses and detection.

Corresponding author: Jianqiang Hu

Cite this article
Xiaohua Chen, Huan Guo, Jing Yi and Jianqiang Hu, Fabrication of AucoreCo3O4shell/PAA/HRP Composite Film for Direct Electrochemistry and Hydrogen Peroxide Sensor Applications, Sens. Mater., Vol. 21, No. 8, 2009, p. 433-444.

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