In this study, we present a kinetic investigation of interfacial cardiac troponin I (cTnI)-aptamer interactions using cyclic voltammetry (CV). An aptamer-complementary strand complex immobilized on a gold electrode was employed as a model system to examine the concentration-dependent adsorption behavior of cTnI. CV measurements with respect to the interaction time revealed that the interaction process follows pseudo-first-order kinetics under constant surface coverage conditions. By correlating charge variation with fractional surface occupation, an interaction rate constant (k) was derived from the kinetic model. A strong power-law relationship (R² = 0.9515) between cTnI concentration and the derived rate constant was established, demonstrating a predictable concentration-dependent detection.