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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 23, Number 4 (2011)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 219-228
S&M840 Research Paper of Special Issue https://doi.org/10.18494/SAM.2011.728 Published: July 11, 2011 DNA-Mediated Sensitive Detection and Quantification of Rare Earth Ions Using Polymerase Chain Reaction [PDF] Ken Yokawa, Takashi Kadono, Yusuke Suzuki, Takuya Suzuki, Kazuya Uezu and Tomonori Kawano (Received October 1, 2010; Accepted November 16, 2010) Keywords: biosensing, DNA, lanthanum PCR, rare earth, real-time PCR
In this article, a novel approach to a highly sensitive and quantitative detection of rare earth ions by the polymerase chain reaction (PCR) technique is described, by focusing on the catalytic activities of rare earth ions towards deoxyribonucleic acid (DNA). The detection of rare earth ions is based on the observation that rare earth ions such as La3+ act against PCR-dependent amplification of DNA. In addition to the detection of rare earth ions by the conventional PCR protocol with agarose gel elecrophoresis, we also quantified rare earth ions by real-time PCR. Finally, our approach using PCR greatly lowered the limit for the biochemical detection of rare earth ions at the ppb level.
Corresponding author: Tomonori Kawano Cite this article Ken Yokawa, Takashi Kadono, Yusuke Suzuki, Takuya Suzuki, Kazuya Uezu and Tomonori Kawano, DNA-Mediated Sensitive Detection and Quantification of Rare Earth Ions Using Polymerase Chain Reaction, Sens. Mater., Vol. 23, No. 4, 2011, p. 219-228. |
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