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Vol. 34, No. 8(3), S&M3042

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Vol. 32, No. 8(2), S&M2292

Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials, Volume 16, Number 1 (2004)
Copyright(C) MYU K.K.
pp. 43-51
S&M546 Research Paper in Biosensors and Related Areas
Published: 2004

Characterization of Ti/Au Metal Spot Arrays by a Wavelength Interrogation-Based Surface Plasmon Resonance Biosensor [PDF]

Jong Seol Yuk, Sun-Ju Yi, Seoung-Woo Park, Jeong-A Han, Young-Myeong Kim and Kwon-Soo Ha

(Received September 9, 2003; Accepted January 5, 2004)

Keywords: surface plasmon resonance, transglutaminase, protein arrays, SPR biosensor

We have investigated the characteristics of surface plasmon resonance (SPR) spectra as a function of metal thickness for the application of protein arrays by using a wavelength interrogation-based SPR biosensor (self-developed). Resonance intensity and wavelength were significantly affected by the thickness of Au film. Minimal resonance intensity was observed at a thickness of approximately 450 Å of the Au film and changed slightly according to the incidence angle. A Ti film as an adhesion layer had a significant influence on the half-width of the SPR spectrum and the resonance wavelength, and a 50Å-thick Ti layer under the 450Å-thick Au film yielded a good spectrum in these experiments. In addition, the interaction of anti-tissue transglutaminase and tissue transglutaminase was successfully analyzed on the array of Ti/Au (50 Å /450 Å) metal spots by using the wavelength interrogation-based SPR biosensor. Thus, it is important to consider the optimal thickness of the Au film and Ti adhesion layer when biospecific interactions are analyzed on protein arrays by SPR biosensors.

Corresponding author: Kwon-Soo Ha


Cite this article
Jong Seol Yuk, Sun-Ju Yi, Seoung-Woo Park, Jeong-A Han, Young-Myeong Kim and Kwon-Soo Ha, Characterization of Ti/Au Metal Spot Arrays by a Wavelength Interrogation-Based Surface Plasmon Resonance Biosensor, Sens. Mater., Vol. 16, No. 1, 2004, p. 43-51.



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