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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 27, Number 5 (2015)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 403-411
S&M1079 Research Paper of Special Issue https://doi.org/10.18494/SAM.2015.1180 Published: June 8, 2015 Rapid Quantitative Detection by Real-Time Polymerase Chain Reaction with a Polymer Chip [PDF] Yoshinori Akagi, Kazuhiko Imamura, Takamasa Kouno, Shigeru Nomura, Shuji Kage, Mitsuyoshi Ema, Naoki Nishijima, Kazuki Yamamoto, Toshiro Migita and Futoshi Shibasaki (Received February 24, 2015; Accepted April 13, 2015) Keywords: PCR, DNA amplification, array chip, polymer chip, rapid PCR
The conventional real-time polymerase chain reaction (PCR) used in clinical settings is slow (>1 h). We describe the development of a real-time PCR system for the rapid, high sensitivity detection of infectious diseases. We have improved the polymer-based PCR chip and thermal control unit. A polymer with high thermal diffusivity, thermal conductivity, and glass transition temperature was used for the PCR chip. The thermal control unit consisted of a denaturing block, an annealing/extension block, and a cooling block set at 20 ℃. The heating and cooling rates were 7.2 and −14.2 ℃/s, respectively. The cooling rate was 10 times higher with the cooling block than without the cooling block. Using this rapid real-time PCR system, we sucessfully detected mycoplasma genomic deoxyribonucleic acid with a detection limit of 6.0 × 10−5 ng/µL in 8.5 min.
Corresponding author: Futoshi Shibasaki Cite this article Yoshinori Akagi, Kazuhiko Imamura, Takamasa Kouno, Shigeru Nomura, Shuji Kage, Mitsuyoshi Ema, Naoki Nishijima, Kazuki Yamamoto, Toshiro Migita and Futoshi Shibasaki, Rapid Quantitative Detection by Real-Time Polymerase Chain Reaction with a Polymer Chip, Sens. Mater., Vol. 27, No. 5, 2015, p. 403-411. |
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