pp. 593-599
S&M1213 Research Paper of Special Issue https://doi.org/10.18494/SAM.2016.1277 Published: June 22, 2016 Micro-Polymerase Chain Reaction Chip Reaction Chamber Temperature Measurement [PDF] Chan-Young Park, Seung-Cheol Lee, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim (Received March 1, 2015; Accepted January 14, 2016) Keywords: microfluidic channel, internal temperature, polymerase chain reaction, NTC-thermistor, thermocouple
In this paper, we discuss the correlation between the reagent temperature and the thermistor sensor outside the chamber. The polymerase chain reaction (PCR) chip employed is constructed on a printed circuit board (PCB) with a heating pattern and a thermistor at the opposite side of the chamber. Since the reaction chamber is made with various films and tapes, a thermocouple could be embedded easily to measure the reagent temperature within the chamber. The parameters for chip control, such as the maximum heating power and the temperature compensation coefficient, were determined using the measured temperatures. The results show that the parameters determined enabled the exact control of the reagent temperature during the polymerase chain reaction.
Corresponding author: Jong-Dae KimCite this article Chan-Young Park, Seung-Cheol Lee, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim, Micro-Polymerase Chain Reaction Chip Reaction Chamber Temperature Measurement, Sens. Mater., Vol. 28, No. 6, 2016, p. 593-599. |