pp. 961-968
S&M1386 Research Paper of Special Issue https://doi.org/10.18494/SAM.2017.1548 Published: July 26, 2017 Calibration of Temperature Sensors with Different Tolerances for Polymerase Chain Reaction Chips [PDF] Chan-Young Park, Seul-Bit-Na Koo, Jae-Hyeon Cho, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim (Received February 26, 2016; Accepted February 27, 2017) Keywords: micro-PCR chip, calibration, thermistor, resistance tolerance, PCR performance
The calibration of temperature sensors with different tolerances is investigated to evaluate the effect of a polymerase chain reaction (PCR) on the performance of a PCR chip. Two types of thermistor sensors with 0.5 and 1% tolerances are applied to printed circuit board (PCB)-based PCR chips. The PCR performance is evaluated for calibrated and uncalibrated thermistors. The band volume variation of gel images is analyzed statistically to evaluate the performance. As a result of analyzing the band volume in gel images, the 0.5% thermistor was found to exhibit a level of performance that is almost equal in both the calibrated and uncalibrated thermistors.
Corresponding author: Jong-Dae KimCite this article Chan-Young Park, Seul-Bit-Na Koo, Jae-Hyeon Cho, Yu-Seop Kim, Hye-Jeong Song, and Jong-Dae Kim, Calibration of Temperature Sensors with Different Tolerances for Polymerase Chain Reaction Chips, Sens. Mater., Vol. 29, No. 7, 2017, p. 961-968. |