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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 29, Number 5 (2017)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 567-573
S&M1350 Research Paper of Special Issue https://doi.org/10.18494/SAM.2017.1559 Published: May 10, 2017 Development of a Bead-Based Multiplexed Immunoassay Using Image Cytometric Analysis [PDF] Yoshiaki Ukita, Chiwa Kataoka, Kazuyuki Sawadaishi, Akinobu Yamaguchi, and Yuichi Utsumi (Received December 13, 2016; Accepted March 9, 2017) Keywords: multiplexed immunoassay image cytometry, bead-based assay
In this paper, we present the demonstration of a size-encoded bead-based multiplexed immunoassay with image cytometric decoding and readout. Antibodies were immobilized on polystyrene microspheres by physical adsorption and employed for the demonstration of this duplexed immunoassay. First, we carried out a duplexed immunoassay with a mouse and rat IgG detection system and discussed the problem of cross-reactivity inherent to these immunoreaction systems. We then carried out a duplexed immunoassay with a mouse IgG and human albumin detection system, which was less cross-reactive. We subsequently demonstrated the application of size-encoded image cytometric decoding and the detection of mouse IgG and human albumin using this duplexed immunoassay system.
Corresponding author: Yoshiaki Ukita Cite this article Yoshiaki Ukita, Chiwa Kataoka, Kazuyuki Sawadaishi, Akinobu Yamaguchi, and Yuichi Utsumi, Development of a Bead-Based Multiplexed Immunoassay Using Image Cytometric Analysis, Sens. Mater., Vol. 29, No. 5, 2017, p. 567-573. |
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