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S&M2685 Research Paper https://doi.org/10.18494/SAM.2021.3583 Published: September 24, 2021 Stable Immobilization of Mediator in Porous Carbon Material via Capping Technique for Fabricating Sensitive Enzyme-based Glucose Sensor [PDF] Kuniaki Nagamine, Ryota Izawa, Isao Shitanda, and Shizuo Tokito (Received August 11, 2021; Accepted September 8, 2021) Keywords: porous carbon, enzyme, biosensor, electrochemistry, capping technique
A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.
Corresponding author: Kuniaki Nagamine, Shizuo TokitoThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Kuniaki Nagamine, Ryota Izawa, Isao Shitanda, and Shizuo Tokito, Stable Immobilization of Mediator in Porous Carbon Material via Capping Technique for Fabricating Sensitive Enzyme-based Glucose Sensor, Sens. Mater., Vol. 33, No. 9, 2021, p. 3245-3253. |