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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials
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Sensors and Materials, Volume 33, Number 9(3) (2021)
Copyright(C) MYU K.K.
pp. 3245-3253
S&M2685 Research Paper
https://doi.org/10.18494/SAM.2021.3583
Published: September 24, 2021

Stable Immobilization of Mediator in Porous Carbon Material via Capping Technique for Fabricating Sensitive Enzyme-based Glucose Sensor [PDF]

Kuniaki Nagamine, Ryota Izawa, Isao Shitanda, and Shizuo Tokito

(Received August 11, 2021; Accepted September 8, 2021)

Keywords: porous carbon, enzyme, biosensor, electrochemistry, capping technique

A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.

Corresponding author: Kuniaki Nagamine, Shizuo Tokito


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Cite this article
Kuniaki Nagamine, Ryota Izawa, Isao Shitanda, and Shizuo Tokito, Stable Immobilization of Mediator in Porous Carbon Material via Capping Technique for Fabricating Sensitive Enzyme-based Glucose Sensor, Sens. Mater., Vol. 33, No. 9, 2021, p. 3245-3253.



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