Young Researcher Paper Award 2023
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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 34, Number 3(1) (2022)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 951-960
S&M2865 Research Paper of Special Issue https://doi.org/10.18494/SAM3598 Published: March 3, 2022 A Measurement Method for Cytochrome P450 3A4 (CYP3A4)-mediated Oxidation of Cholesterol in Lipid Membranes [PDF] Atsushi Shoji, Haruka Miki, Mikiko Kikkawa, Miyu Yamamoto, Yukiko Moriiwa, Kazuhiro Morioka, and Akio Yanagida (Received August 24, 2021; Accepted February 8, 2022) Keywords: streptolysin O, CYP3A4, liposome, erythromycin
We have developed a measurement method for the cytochrome P450 3A4 (CYP3A4)-mediated oxidation of cholesterol in lipid membranes. This method is based on the sterol-structural selectivity for the nanopore formation of streptolysin O (SLO) in a lipid membrane. A decrease in the fraction of 4β-hydroxycholesterol in lipid membranes decreased the nanopore forming ability of SLO. The incubation of the liposomes consisting of l-α-phosphatidylcholine (PC), l-α-phosphatidylethanolamine (PE), and cholesterol (0.98:0.02:0.75, mol/mol) with CYP3A4 decreased the nanopore forming ability of SLO, indicating the conversion of cholesterol to 4β-hydroxycholesterol. The change in nanopore forming ability did not occur in the presence of erythromycin, which is an inhibitor of CYP3A4.
Corresponding author: Atsushi Shoji  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Atsushi Shoji, Haruka Miki, Mikiko Kikkawa, Miyu Yamamoto, Yukiko Moriiwa, Kazuhiro Morioka, and Akio Yanagida, A Measurement Method for Cytochrome P450 3A4 (CYP3A4)-mediated Oxidation of Cholesterol in Lipid Membranes, Sens. Mater., Vol. 34, No. 3, 2022, p. 951-960. |
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