pp. 3173-3183
S&M3033 Research Paper of Special Issue https://doi.org/10.18494/SAM3914 Published: August 15, 2022 Development of Small-sized Lysine Enzyme Sensor for Clinical Use [PDF] Takeshi Uemura, Shunsuke Fujii, Hiroki Yamazaki, Tetsuji Itoh, Kouji Masumoto, and Seiichi Nishizawa (Received March 31, 2022; Accepted July 6, 2022) Keywords: lysine, enzyme sensor, amperometric sensor
We have developed an electrochemical biosensor to measure the lysine concentration in blood. The lysine biosensor has a simple design and can complete measurements of a 5 μL sample in 2 min, enabling rapid measurement in clinical practice. The target molecules are catalyzed by enzymes encapsulated in carboxymethyl cellulose on the working electrodes to produce potassium ferrocyanide as an electron mediator that is detected on the electrodes amperometrically. In a clinical application, the sensor was used to measure blood lysine concentrations in 47 pediatric surgical patients. The results showed that the lysine level was correlated with the transthyretin level, a conventional nutritional indicator, confirming its usefulness for nutritional assessment. This sensor can be used for postoperative nutritional management and treatment of rare diseases such as lysinuric protein intolerance. The platform also has a versatile design for the detection of other amino acids in addition to lysine.
Corresponding author: Kouji Masumoto, Seiichi NishizawaThis work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Takeshi Uemura, Shunsuke Fujii, Hiroki Yamazaki, Tetsuji Itoh, Kouji Masumoto, and Seiichi Nishizawa, Development of Small-sized Lysine Enzyme Sensor for Clinical Use, Sens. Mater., Vol. 34, No. 8, 2022, p. 3173-3183. |