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Vol. 32, No. 8(2), S&M2292

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Sensors and Materials, Volume 36, Number 9(4) (2024)
Copyright(C) MYU K.K.
pp. 4101-4110
S&M3787 Research Paper of Special Issue
https://doi.org/10.18494/SAM5051
Published: September 30, 2024

Development of a Portable Loop-mediated Isothermal Amplification Device for DNA Detection and Determination Using Absorbance Measurement [PDF]

Zhuo Huang, Shoji Yamamoto, Yifan Liao, Kazuhiro Morioka, and Hizuru Nakajima

(Received March 25, 2024; Accepted July 29, 2024)

Keywords: absorbance detection, LAMP, PTC heater, on-site nucleic acid test, portable device

A portable multisample absorbance detection system for loop-mediated isothermal amplification using a positive temperature coefficient (PTC) heater was developed in this study. The system can simultaneously measure up to four samples and consists of four LEDs, four photodiodes, two PTC heaters, a sample holder, and a short-pass filter. The system successfully differentiated samples with and without human genomic DNA with photodiodes measuring absorbance in real time. We have also demonstrated that the quantitative analysis of human genomic DNA is possible at initial DNA concentrations ranging from 2.11 × 102 to 2.11 × 104 ng/µL. This device has potential use in on-site genetic testing owing to its compact size, portability, and capability to provide rapid results.

Corresponding author: Shoji Yamamoto and Hizuru Nakajima


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Cite this article
Zhuo Huang, Shoji Yamamoto, Yifan Liao, Kazuhiro Morioka, and Hizuru Nakajima, Development of a Portable Loop-mediated Isothermal Amplification Device for DNA Detection and Determination Using Absorbance Measurement, Sens. Mater., Vol. 36, No. 9, 2024, p. 4101-4110.



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