Young Researcher Paper Award 2023
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Print: ISSN 0914-4935
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
Online: ISSN 2435-0869
Sensors and Materials
is an international peer-reviewed open access journal to provide a forum for researchers working in multidisciplinary fields of sensing technology.
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Sensors and Materials
is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
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Sensors and Materials
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is covered by Science Citation Index Expanded (Clarivate Analytics), Scopus (Elsevier), and other databases.
Instructions to authors
English 日本語
Instructions for manuscript preparation
English 日本語
Template
English
Publisher
MYU K.K.
Sensors and Materials
1-23-3-303 Sendagi,
Bunkyo-ku, Tokyo 113-0022, Japan
Tel: 81-3-3827-8549
Fax: 81-3-3827-8547
MYU Research, a scientific publisher, seeks a native English-speaking proofreader with a scientific background. B.Sc. or higher degree is desirable. In-office position; work hours negotiable. Call 03-3827-8549 for further information.
MYU Research
(proofreading and recording)
MYU K.K.
(translation service)
The Art of Writing Scientific Papers
(How to write scientific papers)
(Japanese Only)
Sensors and Materials, Volume 36, Number 9(4) (2024)
Copyright(C) MYU K.K.
Copyright(C) MYU K.K.
|
pp. 4101-4110
S&M3787 Research Paper of Special Issue https://doi.org/10.18494/SAM5051 Published: September 30, 2024 Development of a Portable Loop-mediated Isothermal Amplification Device for DNA Detection and Determination Using Absorbance Measurement [PDF] Zhuo Huang, Shoji Yamamoto, Yifan Liao, Kazuhiro Morioka, and Hizuru Nakajima (Received March 25, 2024; Accepted July 29, 2024) Keywords: absorbance detection, LAMP, PTC heater, on-site nucleic acid test, portable device
A portable multisample absorbance detection system for loop-mediated isothermal amplification using a positive temperature coefficient (PTC) heater was developed in this study. The system can simultaneously measure up to four samples and consists of four LEDs, four photodiodes, two PTC heaters, a sample holder, and a short-pass filter. The system successfully differentiated samples with and without human genomic DNA with photodiodes measuring absorbance in real time. We have also demonstrated that the quantitative analysis of human genomic DNA is possible at initial DNA concentrations ranging from 2.11 × 102 to 2.11 × 104 ng/µL. This device has potential use in on-site genetic testing owing to its compact size, portability, and capability to provide rapid results.
Corresponding author: Shoji Yamamoto and Hizuru Nakajima  This work is licensed under a Creative Commons Attribution 4.0 International License. Cite this article Zhuo Huang, Shoji Yamamoto, Yifan Liao, Kazuhiro Morioka, and Hizuru Nakajima, Development of a Portable Loop-mediated Isothermal Amplification Device for DNA Detection and Determination Using Absorbance Measurement, Sens. Mater., Vol. 36, No. 9, 2024, p. 4101-4110. |
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