A taste sensor chip miniaturized on the basis of the one-chip-size receptor part of a conventional taste sensor, which can measure taste using lipid/polymer membranes, was fabricated. The reference electrode in the taste sensor chip realized the miniaturization and stabilization of potential using a multilayer structure of a pHEMA layer and other polymer layers. In this study, we improved the stability of the taste sensor chip during the measurement by changing the material of the partition of the taste sensor chip to plastic. We successfully integrated the working and reference electrodes on the chip. The portable taste sensor system was composed of the taste sensor chip, an integrated electric signal processing part and a laptop computer. We considered that the design of the portable taste sensor facilitates the easy manual measurement of taste. By realizing the taste sensor chip and the portable taste sensor, taste measurement can be easily performed anywhere.