The mixed-flow production line has realized a production method featuring multiple varieties and small batches, retaining the advantages of large scale, high efficiency, and low cost of traditional assembly line production. The mixed-flow production line enhances production flexibility but also escalates the cost and demands of production management for the enterprise. To efficiently process a variety of products in small batches on the same assembly line, the issue of production sequencing for different products on the mixed-flow assembly line needs to be addressed. In this study, we utilize the FlexSim platform to simulate the workshop production activities of J Machinery Factory over one production cycle. By establishing a model and employing the OPTQUEST optimization module, the optimal solution is rapidly determined without increasing machine equipment, thereby maximizing total output profit. This provides a scientific quantitative basis for enterprise management and decision-making, ultimately enhancing competitiveness.