pp. 299-308
S&M727 Research Paper of Special Issue https://doi.org/10.18494/SAM.2008.547 Published: December 4, 2008 Application of Genetically Engineered Acetylcholinesterases in Screen-Printed Amperometric Biosensor for Detection of Organophosphorus Insecticides [PDF] Angelika Galezowska, Tomasz Sikora, Georges Istamboulie, Marek Trojanowicz, Iwona Polec, Gilvanda Silva Nunes, Thierry Noguer and Jean-Louis Marty (Received December 15, 2008; Accepted December 15, 2008) Keywords: organophosphorus pesticide, amperometric biosensor, screen-printed electrode, acetylcholinesterase
The inhibition sensitivity of wild and mutant acetylcholinesterases (AChE) towards selected organophosphorus pesticides has been compared with enzymes immobilized in a photocured layer of polyvinylalcohol polymer (PVA-AWP) on a screen-printed graphite electrode. The investigated pesticides included the widely used malaoxon (MO), chlorfenvinphos (CFV), and chlorpyriphos-oxon (CPO). The last two insecticides are in the EC priority list of toxic compounds to be detected in water. The limits of detection (LOD) obtained with each pesticide tested were in accordance with the European regulation.
Cite this article Angelika Galezowska, Tomasz Sikora, Georges Istamboulie, Marek Trojanowicz, Iwona Polec, Gilvanda Silva Nunes, Thierry Noguer and Jean-Louis Marty, Application of Genetically Engineered Acetylcholinesterases in Screen-Printed Amperometric Biosensor for Detection of Organophosphorus Insecticides, Sens. Mater., Vol. 20, No. 6, 2008, p. 299-308. |