pp. 193-200
S&M801 Research Paper of Special Issue https://doi.org/10.18494/SAM.2010.641 Published: June 10, 2010 Highly Sensitive Detection of TNT Using a Poly(amidoamine) Dendron-Based SPR Immunosensor [PDF] Yutaka Mizuta, Takeshi Onodera, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko (Received October 5, 2009; Accepted April 13, 2010) Keywords: SPR, TNT, PAMAM, dendron, hapten
In this paper, we describe the fabrication of novel poly(amidoamine) (PAMAM) dendron-based Surface Plasmon Resonance (SPR) sensor chip surfaces supported by a self-assembled monolayer (SAM) of alkanethiol and their use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is one of main components of explosives. The fabricated surfaces showed excellent binding capacity for TNT antibodies; this is probably due to the fact that the highly branched functional groups and three-dimensional structure of dendron molecules offer densely arranged binding sites and improve binding efficiency. A limit of detection (LOD) of ca. 15 ppt (pg ml−1) was achieved by inhibition assay.
Corresponding author: Yutaka MizutaCite this article Yutaka Mizuta, Takeshi Onodera, Praveen Singh, Kiyoshi Matsumoto, Norio Miura and Kiyoshi Toko, Highly Sensitive Detection of TNT Using a Poly(amidoamine) Dendron-Based SPR Immunosensor, Sens. Mater., Vol. 22, No. 4, 2010, p. 193-200. |