pp. 881-889
S&M1124 Research Paper of Special Issue https://doi.org/10.18494/SAM.2015.1196 Published: October 7, 2015 Microscale Chemical and Enzyme Sensor Arrays Prepared by Microcontact Printing [PDF] Masayasu Suzuki, Akitaka Nomura, Mizuki Yamamoto, Kenta Minakuchi and Yasunori Iribe (Received April 1, 2015; Accepted June 29, 2015) Keywords: microcontact printing, pH sensor, oxygen sensor, glucose sensor, lactate sensor
Fluorescence-based integrated chemical and enzyme sensor arrays on which two kinds of 10 µm inside diameter (i.d.) sensor spots were arranged alternately on one chip were developed using microcontact printing. Fluorescein isothiocyanate (FITC), a ruthenium complex, and europium tetracycline (EuTc) were stamped onto diamond-like carbon (DLC) sputtered glass slides for pH, oxygen, and hydrogen peroxide sensors, respectively. After printing oxygen sensor spots, pH sensor spots were stamped between the oxygen sensor spots using the same polydimethylsiloxane (PDMS) stamp. In the same way, glucose oxidase and lactate oxidase spots were stamped in an alternating pattern onto H2O2 sensor spots. Each sensor in the integrated sensor showed specific responses independently.
Corresponding author: Masayasu SuzukiCite this article Masayasu Suzuki, Akitaka Nomura, Mizuki Yamamoto, Kenta Minakuchi and Yasunori Iribe, Microscale Chemical and Enzyme Sensor Arrays Prepared by Microcontact Printing, Sens. Mater., Vol. 27, No. 9, 2015, p. 881-889. |